Chidule | Kuzindikira zenizeniMatenda a Mapazi ndi Pakamwa A antibody |
Mfundo yofunika | Gulu la FMD Antibody ELISA test kit limagwiritsa ntchito kuzindikira ma antibodies a matenda a phazi ndi pakamwa mu seramu ya nkhumba, ng'ombe, nkhosa ndi mbuzi poyesa chitetezo cha katemera wa FMD. |
Zolinga Zozindikira | Matenda a Mapazi ndi Pakamwa Mtundu A Antibody |
Chitsanzo | Seramu
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Kuchuluka | 1 kit = 192 Mayeso |
Kukhazikika ndi Kusunga | 1) Ma reagents onse ayenera kusungidwa pa 2 ~ 8 ℃.Osaundana. 2) Nthawi ya alumali ndi miyezi 12.Gwiritsani ntchito ma reagents onse tsiku lomaliza ntchito lisanafike.
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Kachilombo ka matenda a phazi ndi mkamwa(FMDV) ndiyetizilombo toyambitsa matendazomwe zimayambitsamatenda a phazi ndi mkamwa. Ndi apicornavirus, membala wofananira wamtunduAphthovirus.Matendawa, amene amayambitsa vesicles (matuza) m`kamwa ndi mapazi ang'ombe, nkhumba, nkhosa, mbuzi, ndi zinawa ziboda zapakatinyama ndi matenda opatsirana kwambiri ndi mliri waukulu waulimi wa ziweto.
Chidachi chimagwiritsa ntchito njira yampikisano ya ELISA polimbana ndi ma antigen a matenda a phazi ndi pakamwa pazitsime za microplate.Poyesa, onjezerani kuchepetsedwa kwa seramu chitsanzo ndi odana ndi FMD Ab, pambuyo makulitsidwe, ngati pali FMD antibody, izo kuphatikiza ndi chisanadze TACHIMATA antigen, antibody mu chitsanzo chipika kuphatikiza odana ndi FMD antibody ndi chisanadze TACHIMATA antigen;kutaya enzyme yosakanikirana ndi kuchapa;Onjezani gawo laling'ono la TMB mu zitsime zazing'ono, chizindikiro cha buluu chopangidwa ndi Enzyme catalysis chili mugawo losiyana la zomwe zili mu zitsanzo.
Kachilombo ka matenda a phazi ndi mkamwazimachitika zazikulu zisanuserotypes: O, A, C, SAT-1, SAT-2, SAT-3, ndi Asia-1.Ma serotypes awa amawonetsa madera ena, ndipo serotype ya O ndiyofala kwambiri.
Reagent | Voliyumu 96 Mayesero/192Mayeso | ||
1 |
| 1 ndi/2e | |
2 |
| 2.0ml ku | |
3 |
| 1.6ml pa | |
4 |
| 100 ml | |
5 |
| 100 ml | |
6 |
| 11/22 ml | |
7 |
| 11/22 ml | |
8 |
| 15ml ku | |
9 |
| 2 ndi/4e | |
10 | seramu dilution microplate | 1 ndi/2e | |
11 | Malangizo | 1 pcs |