Nambala yakatalogi | Mtengo wa RC-CF28 |
Chidule | Kuzindikira ma antibodies a anti-Toxoplasma IgG/IgM mkati mwa mphindi 10 |
Mfundo yofunika | Gawo limodzi la immunochromatographic assay |
Zolinga Zozindikira | Toxoplasma IgG/IgM antibody |
Chitsanzo | Feline Magazi Onse, Plasma kapena Seramu |
Nthawi yowerenga | 10-15 mphindi |
Kumverera | IgG : 97.0 % vs. IFA , IgM : 100.0 % vs. IFA |
Mwatsatanetsatane | IgG : 96.0 % vs. IFA , IgM : 98.0 % vs. IFA |
Kuchuluka | 1 bokosi (zida) = 10 zida (payekha payokha) |
Zamkatimu | Zida zoyesera, botolo la Buffer, ndi zotsitsa zotayidwa |
Kusungirako | Kutentha kwachipinda (2 ~ 30 ℃) |
Kutha ntchito | Miyezi 24 pambuyo popanga |
Chenjezo | Gwiritsani ntchito mkati mwa mphindi 10 mutatsegulaGwiritsani ntchito sampuli yoyenera (0.01 ml ya dropper) Gwiritsani ntchito pambuyo pa mphindi 15-30 ku RT ngati zasungidwa m'malo ozizira Ganizirani zotsatira za mayeso ngati zosavomerezeka pakatha mphindi 10 |
Toxoplasmosis ndi matenda omwe amayamba chifukwa cha tizilombo toyambitsa matenda tomwe timatchedwa Toxoplasma gondii (T.gondii).Toxoplasmosis ndi imodzi mwa matenda ofala kwambiri a parasitic ndipo amapezeka pafupifupi nyama zonse zotentha, kuphatikizapo ziweto ndi anthu.Amphaka ndi ofunikira pa miliri ya T. gondii chifukwa ndi okhawo omwe amatha kutulutsa ma oocysts osagwirizana ndi chilengedwe.Amphaka ambiri omwe ali ndi T.gondii sawonetsa zizindikiro zilizonse.Komabe, nthawi zina, matenda a toxoplasmosis amapezeka.Matenda akachitika, amatha kukula pamene chitetezo cha mthupi cha mphaka sichikwanira kuti asiye kufalikira kwa mitundu ya tachyzoite.Matendawa amapezeka kwambiri amphaka omwe ali ndi chitetezo chamthupi choponderezedwa, kuphatikizapo ana amphaka ndi amphaka omwe ali ndi kachilombo ka khansa ya m'magazi (FELV) kapena feline immunodeficiency virus (FIV).
Amphaka ndiwo okhawo omwe amalandila T.gondii;ndizo zoyamwitsa zokha zomwe Toxoplasma imadutsa mu ndowe.Mphaka, mawonekedwe oberekera a T.gondii amakhala m'matumbo ndipo ma oocysts (ofanana ndi mazira osakhwima) amatuluka m'thupi mu ndowe.Ma oocysts ayenera kukhala m'malo 1-5 masiku asanatenge kachilombo.Amphaka amangodutsa T.gondii mu ndowe zawo kwa milungu ingapo atadwala.Ma oocysts amatha kukhala ndi moyo kwa zaka zingapo m'chilengedwe ndipo sagonjetsedwa ndi mankhwala ambiri ophera tizilombo.
Ma oocysts amalowetsedwa ndi makamu apakatikati monga makoswe ndi mbalame, kapena nyama zina monga agalu ndi anthu, ndipo zimasamukira ku minofu ndi ubongo.Pamene mphaka amadya kachilombo wapakatikati nyama (kapena mbali yanyama yaikulu, mwachitsanzo, nkhumba), tizilombo toyambitsa matenda timatuluka m'matumbo a mphaka ndipo moyo ukhoza kubwerezedwa.
Ambiri zizindikiro zatoxoplasmosis monga malungo, kusowa chilakolako cha chakudya, ndi ulesi.Zizindikiro zina zimatha kutengera ngati matendawa ndi owopsa kapena osatha, komanso komwe tizilomboto timapezeka m'thupi.M'mapapo, matenda a T.gondii angayambitse chibayo, chomwe chimayambitsa kupuma kwapang'onopang'ono.Toxoplasmosis imatha kukhudzanso maso ndi dongosolo lapakati lamanjenje, kupangitsa kutupa kwa retina kapena chipinda cham'mbuyo chamkati, kukula kwa mwana wosabadwayo komanso kuyankha kwa kuwala, khungu, kusagwirizana, kukhudzika kwa kukhudza, kusintha kwa umunthu, kuzungulira, kukanikiza mutu, kugwedezeka kwa makutu. , kuvutika kutafuna ndi kumeza chakudya, kukomoka, ndi kulephera kudziletsa pokodza ndi kuchita chimbudzi.
Toxoplasmosis nthawi zambiri imapezeka potengera mbiri, zizindikiro za matenda, ndi zotsatira za mayeso othandizira a labotale.Kuyeza kwa ma antibodies a IgG ndi IgM ku Toxoplasma gondii m'magazi kungathandize kuzindikira toxoplasmosis.Kukhalapo kwa ma antibodies ofunikira a IgG kwa T.gondii mu mphaka wathanzi kukuwonetsa kuti mphaka adadwalapo kale ndipo tsopano ali ndi chitetezo chodziteteza komanso osatulutsa ma oocysts.Kupezeka kwa ma antibodies a IgM ku T.gondii, komabe, kumasonyeza kuti mphaka ali ndi matenda.Kusapezeka kwa ma antibodies a T.gondii a mitundu yonse iwiri pa mphaka wathanzi kumasonyeza kuti mphaka amatha kutenga matenda ndipo motero amataya maocysts kwa sabata imodzi kapena iwiri atadwala.
Palibe katemera mpaka pano woteteza matenda a T.gondii kapena toxoplasmosis mwa amphaka, anthu, kapena mitundu ina.Choncho, mankhwala nthawi zambiri amakhala ndi mankhwala otchedwa clindamycin.Mankhwala ena omwe amagwiritsidwa ntchito ndi pyrimethamine ndi sulfadiazine, omwe amagwira ntchito limodzi kuti aletse kubereka kwa T.gondii.Kuchiza kuyenera kuyambika msanga atapezeka ndi matendawa ndikupitilira kwa masiku angapo zizindikiro zitatha.
Matenda a pachimake amadziwika ndi kukwera msanga kwa ma antibody a IgM, kutsatiridwa pakadutsa masabata 3-4 ndi kukwera kwa gulu la IgG.Ma antibody a IgM amafika pachimake pafupifupi masabata 3-4 pambuyo poyambira ndipo amakhalabe odziwika kwa miyezi 2-4.Ma antibodies a gulu la IgG amakwera pakadutsa masabata 7-12, koma amatsika pang'onopang'ono kuposa ma antibody a IgM ndipo amakhalabe okwera kwa miyezi 9-12.