Anaplasma Phagocytophilum Ab Test Kit | |
Nambala yakatalogi | Mtengo wa RC-CF26 |
Chidule | Kuzindikira kwa ma antibodies enieni a Anaplasmamkati mwa mphindi 10 |
Mfundo yofunika | Gawo limodzi la immunochromatographic assay |
Zolinga Zozindikira | Ma antibodies a anaplasma |
Chitsanzo | Canine magazi athunthu, seramu kapena plasma |
Nthawi yowerenga | 5-10 mphindi |
Kumverera | 100.0% motsutsana ndi IFA |
Mwatsatanetsatane | 100.0% motsutsana ndi IFA |
Malire Ozindikira | IFA Titer 1/16 |
Kuchuluka | 1 bokosi (zida) = 10 zida (payekha payokha) |
Zamkatimu | Zida zoyesera, botolo la Buffer, ndi zotsitsa zotayidwa |
Chenjezo | Gwiritsani ntchito mkati mwa mphindi 10 mutatsegulaGwiritsani ntchito sampuli yoyenera (0.01 ml ya dropper) Gwiritsani ntchito pambuyo pa mphindi 15-30 ku RT ngati zasungidwa m'malo ozizira Ganizirani zotsatira za mayeso ngati zosavomerezeka pakatha mphindi 10 |
Bakiteriya Anaplasma phagocytophilum (omwe kale anali Ehrilichia phagocytophila) angayambitse matenda mu nyama zingapo kuphatikizapo anthu.Matendawa apezeka m’zoweta za m’nyumba amatchedwanso kuti tick-borne fever (TBF), ndipo akhala akudziwika kwa zaka zosachepera 200.Mabakiteriya a m'banja la Anaplasmataceae ndi gram-negative, nonmotile, coccoid ku ellipsoid zamoyo, kukula kwake kuchokera 0.2 mpaka 2.0um awiri.Ndi ma aerobes ofunikira, alibe njira ya glycolytic, ndipo onse ndi majeremusi ofunikira olowa mu cell.Mitundu yonse yamtundu wa Anaplasma imakhala ndi ma vacuoles okhala ndi nembanemba m'maselo osakhwima kapena okhwima a hematopoietic a mammalian host.Phagocytophilum imayambitsa ma neutrophils ndipo mawu akuti granulocytotropic amatanthauza ma neutrophils omwe ali ndi kachilombo.Nthawi zambiri zamoyo, apezeka eosinophils.
Anaplasma phagocytophilum
Zizindikiro zodziwika bwino za canine anaplasmosis zimaphatikizapo kutentha thupi kwambiri, kutopa, kukhumudwa ndi polyarthritis.Zizindikiro za neurologic (ataxia, khunyu ndi kupweteka kwa khosi) zimatha kuwoneka.Matenda a Anaplasma phagocytophilum sapha kawirikawiri pokhapokha ngati atapangidwa ndi matenda ena.Kutayika kwachindunji, mikhalidwe yopunduka ndi kutayika kwa kupanga kwawonedwa mwa ana ankhosa.Kuchotsa mimba ndi kuwonongeka kwa spermatogenesis mu nkhosa ndi ng'ombe zalembedwa.Kuopsa kwa matendawa kumakhudzidwa ndi zinthu zingapo, monga mitundu yosiyanasiyana ya Anaplasma phagocytophilum yomwe ikukhudzidwa, tizilombo toyambitsa matenda, zaka, chitetezo cha mthupi ndi momwe wodwalayo alili, komanso zinthu monga nyengo ndi kasamalidwe.Ziyenera kutchulidwa kuti mawonetseredwe azachipatala mwa anthu amachokera ku chimfine chochepa chodziletsa, kupita ku matenda owopsa.Komabe, matenda ambiri a anthu mwina amabweretsa zochepa kapena zosawoneka.
Anaplasma phagocytophilum imafalikira ndi nkhupakupa za ixodid.Ku United States mavekita akuluakulu ndi Ixodes scapularis ndi Ixodes pacificus, pamene Ixode ricinus yapezeka kuti ndiyo gwero lalikulu la exophilic ku Ulaya.Anaplasma phagocytophilum imafalitsidwa ndi nkhupakupa za vekitalazi, ndipo palibe umboni wa kufalikira kwa transovarial.Kafukufuku wambiri mpaka pano omwe afufuza za kufunikira kwa makamu a zinyama za A. phagocytophilum ndi tizilombo toyambitsa matenda a nkhupakupa ayang'ana kwambiri makoswe koma chamoyochi chili ndi mitundu yambiri ya zinyama zomwe zimadyetsa amphaka, agalu, nkhosa, ng'ombe, ndi akavalo.
Indirect immunofluorescence assay ndiye mayeso akulu omwe amagwiritsidwa ntchito kuti azindikire matenda.Zitsanzo za acute and convalescent phase serum zitha kuwunikidwa kuti muwone kusintha kowirikiza kanayi kwa antibody titer kukhala Anaplasma phagocytophilum.Ma intracellular inclusions (morulea) amawonedwa mu granulocytes pa Wright kapena Gimsa wopaka magazi opaka magazi.Njira za Polymerase chain reaction(PCR) zimagwiritsidwa ntchito pozindikira DNA ya Anaplasma phagocytophilum.
Palibe katemera woteteza matenda a Anaplasma phagocytophilum.Kupewa kumadalira kupewa kukhudzana ndi nkhupakupa (Ixodes scapularis, Ixodes pacificus, ndi Ixode ricinus) kuyambira masika mpaka kugwa, kugwiritsa ntchito antiacaricides, ndi prophylactic kugwiritsa ntchito doxycycline kapena tetracycline poyendera Ixodes scapularis, Ixodes pacide ricinus, ndi Ixodes madera omwe ali ndi vuto.