Leishmania Ab Test Kit | |
Nambala yakatalogi | Mtengo wa RC-CF24 |
Chidule | Kuzindikira kwa ma antibodies enieni a Leishmaniamkati mwa mphindi 10 |
Mfundo yofunika | Gawo limodzi la immunochromatographic assay |
Zolinga Zozindikira | L. chagasi, L. infantum, ndi L. donovani antiboies |
Chitsanzo | Canine magazi athunthu, seramu kapena plasma |
Nthawi yowerenga | 5-10 mphindi |
Kumverera | 98.9% motsutsana ndi IFA |
Mwatsatanetsatane | 100.0% motsutsana ndi IFA |
Malire Ozindikira | IFA Titer 1/32 |
Kuchuluka | 1 bokosi (zida) = 10 zida (payekha payokha) |
Zamkatimu | Zida zoyesera, botolo la Buffer, ndi zotsitsa zotayidwa |
Kusungirako | Kutentha kwachipinda (2 ~ 30 ℃) |
Kutha ntchito | Miyezi 24 pambuyo popanga |
Chenjezo | Gwiritsani ntchito mkati mwa mphindi 10 mutatsegula Gwiritsani ntchito chitsanzo choyenera (0.01 ml ya dontho) Gwiritsani ntchito pakatha mphindi 15-30 pa RT ngati zasungidwa m'malo ozizira Ganizirani zotsatira za mayeso ngati zosavomerezeka pakatha mphindi 10. |
Leishmaniasis ndi matenda aakulu komanso oopsa a anthu, agalu ndi anyani.Wothandizira wa leishmaniasis ndi tizilombo ta protozoan ndipo ndi wa leishmania donovani complex.Tizilomboti timafala kwambiri m'mayiko otentha komanso otentha ku Southern Europe, Africa, Asia, South America ndi Central America.Leishmania donovani infantum (L. infantum) ndiyomwe imayambitsa matenda a nyani ndi canine ku Southern Europe, Africa, ndi Asia.Canine Leishmaniasis ndi matenda oopsa a systemic.Si agalu onse omwe amayamba kudwala matenda pambuyo pa inoculation ndi tizilombo toyambitsa matenda.Kukula kwa matenda achipatala kumadalira mtundu wa chitetezo cha mthupi chomwe nyama iliyonse imakhala nayo
motsutsana ndi tiziromboti.
Mu Canine
Mawonetseredwe a visceral ndi cutaneous angapezeke nthawi imodzi mwa agalu;mosiyana ndi anthu, ma syndromes osiyana a cutaneous ndi visceral samawoneka.Zizindikiro zachipatala zimakhala zosiyana ndipo zimatha kutsanzira matenda ena.Matenda asymptomatic amathanso kuchitika.Zizindikiro zodziwika bwino za visceral zingaphatikizepo kutentha thupi (komwe kungakhale kwapakatikati), kuchepa kwa magazi m'thupi, lymphadenopathy, splenomegaly, ulesi, kuchepa kwa kulolerana kwa masewera olimbitsa thupi, kuchepa thupi, komanso kuchepa kwa chidwi.Zizindikiro zochepa za visceral zimaphatikizapo kutsegula m'mimba, kusanza, melena, glomerulonephritis,
kulephera kwa chiwindi, epistaxis, polyuria-polydipsia, kuyetsemula, kulumala (chifukwa
polyarthritis kapena myositis), ascites, ndi matenda am'matumbo osatha.
Mu Feline
Amphaka sakhala ndi kachilombo kawirikawiri.M'magulu ambiri amphaka omwe ali ndi kachilomboka, zotupazo zimakhala ndi zilonda zam'mimba, zomwe nthawi zambiri zimapezeka pamilomo, mphuno, zikope, kapena pinnae.Zilonda zam'mimba ndi zizindikiro ndizosowa.
Kuzungulira kwa moyo kumatsirizidwa m'magulu awiri.Mbalame yamsana ndi invertebrate host (sandfly).Ntchentche yaikazi yamchenga imadya ma vertebrates ndipo imameza amastigotes.Ma promastigote okhala ndi mbendera amakula mwa tizilombo.Ma promastigotes amabayidwa mumtundu wa vertebrate pamene akudyetsa mchengawo.Ma promastigotes amakula kukhala amastigotes ndipo amachulukana makamaka mu macrophages.Kuchulukitsa mkati mwa macrophages a
khungu, mucosa ndi viscera, zimayambitsa cutaneous, mucosal ndi visceral leishmaniasis motsatana.
Mwa agalu, leishmaniasis nthawi zambiri imapezeka poyang'anitsitsa tizilombo toyambitsa matenda, pogwiritsa ntchito Giemsa kapena madontho ofulumira, popaka ma lymph node, ndulu, kapena ma aspirates a m'mafupa, ma biopsies a minofu, kapena zotupa pakhungu.Zamoyo zitha kupezekanso mu zotupa zamaso, makamaka mu granulomas.Amastigotes ndi ozungulira kwa tizilombo tozungulira, ndi phata lozungulira basophilic ndi kinetoplast yaing'ono ngati ndodo.Amapezeka mu macrophages kapena amamasulidwa ku maselo osweka.Immunohistochemistry ndi polymerase chain reaction (PCR)
njira zimagwiritsidwanso ntchito.
Mankhwala omwe amagwiritsidwa ntchito kwambiri ndi awa: Meglumine Antimoniate yogwirizana ndi Allopurinol, Aminosidine, ndipo posachedwapa, Amphotericin B. Mankhwalawa amafunikira mlingo wambiri wa mlingo, ndipo izi zidzadalira momwe wodwalayo alili komanso mgwirizano wa mwini wake.Akuti chithandizo chokonzekera chiyenera kusungidwa ndi allopurinol, chifukwa sizingatheke kuonetsetsa kuti agalu sabwereranso ngati chithandizo chatha.Kugwiritsa ntchito makolala okhala ndi mankhwala ophera tizirombo, shamposi kapena zopopera zothandiza kuteteza agalu ku kulumidwa ndi mchenga kuyenera kugwiritsidwa ntchito mosalekeza kwa odwala onse omwe akulandira chithandizo.Kuwongolera ma vector ndi chimodzi mwazinthu zofunika kwambiri pakuwongolera matenda.
Ntchentcheyo imakhala pachiwopsezo ku tizilombo tomwe timapha tizilombo toyambitsa malungo.