Chidule | Kupezeka kwa ma Antibody enieni a matenda a chitopa |
Mfundo yofunika | Chitopa cholimbana ndi matenda a chitopa Elisa chida chimagwiritsidwa ntchito pozindikira chitetezo cha chitopa Virus (NDV) mu seramu, yowunikira ma antibody pambuyo pa chitetezo cha NDVndi serological matenda a matenda mu Avian.
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Zolinga Zozindikira | Matenda a chitopa |
Chitsanzo | Seramu
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Kuchuluka | 1 kit = 192 Mayeso |
Kukhazikika ndi Kusunga | 1) Ma reagents onse ayenera kusungidwa pa 2 ~ 8 ℃.Osaundana. 2) Nthawi ya alumali ndi miyezi 12.Gwiritsani ntchito ma reagents onse tsiku lomaliza ntchito lisanafike.
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Matenda a chitopa ndi matenda opatsirana a avian omwe amakhudza mitundu yambiri ya mbalame zapakhomo ndi zakutchire;imafalikira kwa anthu.Ngakhale imatha kupatsira anthu, nthawi zambiri imakhala yopanda zizindikiro;kawirikawiri zimatha kuyambitsa kutentha thupi pang'ono ndi zizindikiro zonga chimfine komanso/kapena conjunctivitis mwa anthu.Zotsatira zake zimakhala zodziwika kwambiri mu nkhuku zapakhomo chifukwa cha kutengeka kwawo kwambiri komanso kuthekera kowopsa kwa epizootic pamakampani a nkhuku.Ndilofala m’maiko ambiri.Palibe chithandizo chomwe chimadziwika, koma kugwiritsa ntchito katemera wa prophylactic ndi njira zaukhondo kumachepetsa mwayi wa miliri.
Chidachi chimagwiritsa ntchito njira ya ELISA, antigen ya NDV imakutidwa kale pa microplate.Pamene kuyezetsa, kuwonjezera kuchepetsedwa seramu chitsanzo, pambuyo makulitsidwe, ngati pali NDV oteteza enieni, izo kuphatikiza ndi antigen chisanadze TACHIMATA, kutaya osagwirizana antibody ndi zigawo zina ndi kutsuka;Kenako onjezerani enzyme yolembedwa kuti anti-NDV monoclonal antibody, antibody mu chitsanzo chotchinga kuphatikiza kwa anti-monoclonal antibody ndi antijeni wokutidwa kale;kutaya enzyme yosaphatikizana yolumikizana ndi kutsuka. Onjezani gawo laling'ono la TMB mu zitsime zazing'ono, chizindikiro cha buluu chopangidwa ndi Enzyme catalysis chili mugawo losiyana la zomwe zili mu zitsanzo.
Reagent | Voliyumu 96 Mayesero/192Mayeso | ||
1 |
| 1 ndi/2e | |
2 |
| 2.0ml ku | |
3 |
| 1.6ml pa | |
4 |
| 100 ml | |
5 |
| 100 ml | |
6 |
| 11/22 ml | |
7 |
| 11/22 ml | |
8 |
| 15ml ku | |
9 |
| 2 ndi/4e | |
10 | seramu dilution microplate | 1 ndi/2e | |
11 | Malangizo | 1 pcs |