Chidule | Kuzindikira kwa Antibody yeniyeni ya Peste Des Petits Ruminants |
Mfundo yofunika | PPRV antibody ELISA test kit imagwiritsa ntchito kuzindikira ma antibodies a Peste des petits ruminants virus mu seramu ya nkhosa ndi mbuzi. |
Zolinga Zozindikira | PPRV antibody |
Chitsanzo | Seramu
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Kuchuluka | 1 kit = 192 Mayeso |
Kukhazikika ndi Kusunga | 1) Ma reagents onse ayenera kusungidwa pa 2 ~ 8 ℃.Osaundana. 2) Nthawi ya alumali ndi miyezi 12.Gwiritsani ntchito ma reagents onse tsiku lomaliza ntchito lisanafike.
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Ovine rinderpest, yemwe amadziwikanso kutipeste des petits ruminants(PPR), ndi matenda opatsirana omwe amakhudza kwambirimbuzindinkhosa;koma ngamila ndi zazing'ono zakuthengozinyamazingakhudzidwenso.PPR ilipo panoKumpoto, Chapakati, KumadzulondiEast Africa, ndiKuulaya,ndiSouth Asia. Zimayambitsidwa nditizilombo toyambitsa matenda a morbillivirusmu genusMatenda a Morbillivirus,ndipo zimagwirizana kwambiri ndi zina, rinderpest morbillivirus, chikuku morbillivirus,ndicanine morbillivirus(omwe kale ankadziwika kuticaninedistemper virus).Matendawa amapatsirana kwambiri, ndipo amatha kufa ndi 80-100%.pachimakemilandu mu aepizootickukhazikitsa.Kachilomboka sikapatsira anthu.
PPR imadziwikanso kuti mliri wa mbuzi,kata, matenda a stomatitis-pneumoenteritis, ndi ovine rinderpest.
Mabungwe ovomerezeka mongaFAOndiOIEgwiritsani ntchito dzina lachi French"peste des petits ruminants" ndi mitundu ingapo ya masipelo.
Chidachi chimagwiritsa ntchito njira yampikisano ya ELISA kumatira ma antigen a PPRV pazitsime za microplate.Pamene kuyezetsa, kuwonjezera kuchepetsedwa seramu chitsanzo, pambuyo makulitsidwe, ngati PPRV oteteza thupi, izo kuphatikiza ndi antigen pre- TACHIMATA, antibody mu chitsanzo chipika kuphatikiza monoclonal oteteza chitetezo ndi chisanadze TACHIMATA antigen;kutaya enzyme yosakanikirana ndi kuchapa;Onjezani gawo laling'ono la TMB mu zitsime zazing'ono, chizindikiro cha buluu chopangidwa ndi Enzyme catalysis chili mugawo losiyana la zomwe zili mu zitsanzo.
Reagent | Voliyumu 96 Mayesero/192Mayeso | ||
1 |
| 1 ndi/2e | |
2 |
| 2ml ku | |
3 |
| 1.6ml pa | |
4 |
| 100 ml | |
5 |
| 100 ml | |
6 |
| 11/22 ml | |
7 |
| 11/22 ml | |
8 |
| 15ml ku | |
9 |
| 2 ndi/4e | |
10 | seramu dilution microplate | 1 ndi/2e | |
11 | Malangizo | 1 pcs |