Onjezani reagent ku 100ml madzi chitsanzo, mutatha kusungunuka, ikani pa 36 ° C kwa 24h.
Kutanthauzira zotsatira:
colorless = zoipa
Yellow = zabwino zonse za coliforms
Yellow + fluorescence = Escherichia coli positive.
Onjezerani ma reagents ku chitsanzo cha madzi ndikusakaniza bwino.
Thirani mu mbale ya 51-chantitative detector plate (quantitative well plate) kapena 97-well quantitative diagnosis plate (quantitative well plate)
Gwiritsani ntchito makina osindikizira omwe amayendetsedwa ndi pulogalamu
kuti asindikize chimbale chodziwikiratu (quantitative source plate) kuti asindikize ndi kuyika pa 36 ° C kwa 24h.
Chikhalidwe chosamva kutentha kwa coliform/fecal coliform pa 44.5°C kwa 24h ndi chachikasu komanso chabwino.
Kutanthauzira zotsatira:
colorless = zoipa
Yellow checkered = zabwino zonse coliforms
Yellow + fulorosenti grid = Escherichia coli positive reference MPN table count