Zogulitsa
Coliform Gulu Enzvme gawo lapansi kuzindikira reagent Poyesa madzi
Mfundo Yogwirira Ntchito
| Khalidwe | Izi ndi zoyera kapena zopepuka zachikasu |
| Digiri yofotokozera | Zopanda mtundu kapena zachikasu pang'ono |
| PH | 7.0-7.8 |
| Weyiti | 2.7 makilogalamu 0.5 g |
| Kusungirako | Kusunga kwa nthawi yayitali, kuyanika, kusindikiza ndi kupewa kusungirako kuwala pa 4°C - 8°C |
| Nthawi Yovomerezeka | 1 zaka |
| Working Principle | Mu zitsanzo zamadzi zomwe zili ndi mabakiteriya okwana a coliform, mabakiteriya omwe amawatsata adakulira mu ONPG-MUG sing'anga pa 36 土1C. Enzyme yeniyeni ya betagalactosidase yopangidwa ndi mabakiteriya okwana a coliform imatha kuwola gawo lamtundu wa ONPG-MUG sing'anga, zomwe zimapanga. chikhalidwe sing'anga chikasu;Pakadali pano, Escherichia coli imapanga beta-glucuronase yeniyeni kuti iwononge gawo lapansi la fulorosenti MUG mu ONPG-MUG sing'anga ndikupanga mawonekedwe a fulorosenti.Mfundo yomweyi, gulu lolekerera kutentha kwa coliform (gulu la fecal coliform) lidzawola mtundu wa gwero la gawo ONPG mu sing'anga ya ONPG-MUG pa 44.5 土 0 .5 °C, kupanga sing'anga chikasu
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| Kapangidwe kazonyamula | 200 / bokosi |
Njira yogwiritsira ntchito Kuzindikira koyenera
Khwerero 1: Onjezani reagent mu 100ml ya madzi, sungunulani.ndi Chikhalidwe 24h pa 36 士 1 ° C
Gawo2:
Werengani zotsatira zopanda Colorless = zoipa
Yellow = coliform yonse yabwino
Yellow + fluorescence = Escherichia coli positive
Zindikirani: Gulu la coliform lolekerera kutentha (fecal coliform) liyenera kukulitsidwa pa 44.5 士 0 .5°C 24h, ndipo chikasu ndi chabwino.
Kuzindikira kochulukira
1.Add reagent mu 100ml madzi chitsanzo, sungunulani izo.
2.Thirani mu mbale yodziwira dzenje la 51 kapena 97
3. Gwiritsani ntchito LK sealer kuti musindikize mbale yodziwira, ndi kulima 24h pa 36 °C.
4.Results qualitative test idagwiritsidwa ntchito kuti muwone kuchuluka kwa tebulo la MPN.
Zindikirani: Gulu la coliform lolekerera kutentha (gulu la fecal coliform) liyenera kukulitsidwa pa 44.5 士 0.5 ° C 24h, ndi Yellow hole ndi yabwino, ndipo kuwerengera kwa tebulo la MPN kumapezeka.
