1.Add reagent ku 100ml madzi chitsanzo, pambuyo Kutha, incubate pa 36°C kwa 24h.
2. Kutanthauzira zotsatira:
colorless = zoipa
Yellow = zabwino zonse za coliforms
Yellow + fluorescence = Escherichia coli positive.
QUANTlTATlVE DETECTlON
1. Onjezerani ma reagents ku chitsanzo cha madzi ndikusakaniza bwino
2. Thirani mu mbale ya 51-chantitative detector plate (quantitative well plate) kapena 97-well quantitative diagnosis plate (quantitative well plate)
3. Gwiritsani ntchito makina osindikizira omwe amayendetsedwa ndi pulogalamu
kusindikiza diski yodziwira kuchuluka (mbale yachitsime) kuti isindikize ndikuyika pa 36 ° C kwa 24h.
Chikhalidwe chosamva kutentha kwa coliform/fecal coliform pa 44.5°C kwa 24h ndi chachikasu komanso chabwino.
4. Kutanthauzira zotsatira:
colorless = zoipa
Yellow checkered = zabwino zonse coliforms
Yellow + fulorosenti grid = Escherichia coli positive reference MPN table count