Chidule | amagwiritsidwa ntchito pozindikira antigen ya Avian leukosis P27 m'magazi a avian, ndowe, cloaca, ndi dzira loyera. |
Mfundo yofunika | Avian Leukosis (AL) P27 antigen Elisa kit amagwiritsidwa ntchito kuzindikira Avian leukosis P27 antigen m'magazi a mbalame, ndowe, cloaca, ndi dzira loyera.
|
Zolinga Zozindikira | Avian Leukosis (AL) P27 antigen |
Chitsanzo | Seramu
|
Kuchuluka | 1 kit = 192 Mayeso |
Kukhazikika ndi Kusunga | 1) Ma reagents onse ayenera kusungidwa pa 2 ~ 8 ℃.Osaundana. 2) Nthawi ya alumali ndi miyezi 12.Gwiritsani ntchito ma reagents onse tsiku lomaliza ntchito lisanafike.
|
Avian Leukosis (AL) ndi mawu ophatikizidwa a matenda osiyanasiyana okhudzana ndi chotupa mu nkhuku oyambitsidwa ndi Avian Leukosis Virus (ALV) mubanja la Retroviridae.Matendawa amafalitsidwa padziko lonse lapansi ndipo ali ndi chiwerengero chachikulu cha matenda.Zitha kuyambitsa imfa ndi kuwonda kwa nkhuku, kuchepetsa mphamvu zobereketsa ziweto, ndipo ndi imodzi mwa matenda omwe amaika pangozi chitukuko cha nkhuku.Matendawa ali ndi mbiri yakale ndipo nthawi zonse akukumana ndi matenda atsopano, monga gulu la Avian leukemia virus J (ALV-J), lomwe linapezeka ndikudziwika kumapeto kwa zaka za m'ma 1980 ku UK ngati mtundu watsopano wa avian leukemia virus, zomwe zimayambitsa kuwononga makampani a broiler
Chidacho chimagwiritsa ntchito njira ya sangweji ya ELISA, anti-avian leukocyte P27 monoclonal antibody imakutidwa kale pa tinthu tating'onoting'ono ta enzyme. antigen yosamangidwa ndi zigawo zina, enzyme monoclonal antibody imawonjezeredwa kuti imangirire ku antigen-antibody complex pa mbale yoyesera.ndiye kutsuka, conjugate ya enzyme yosamangidwa imachotsedwa, njira ya TMB gawo lapansi imawonjezedwa ku microplate, chizindikiro cha buluu chopangidwa ndi Enzyme catalysis ndi gawo lachindunji la antibody zomwe zili mu zitsanzo.Onjezani njira yoyimitsa, Pambuyo pochitapo kanthu, kuyamwa kwa A phindu pamachitidwewo kumayesedwa ndi kutalika kwa 450 nm.
Reagent | Voliyumu 96 Mayesero/192Mayeso | ||
1 |
| 1 ndi/2e | |
2 |
| 2.0ml | |
3 |
| 1.6ml pa | |
4 |
| 100 ml | |
5 |
| 100 ml | |
6 |
| 11/22 ml | |
7 |
| 11/22 ml | |
8 |
| 15ml | |
9 |
| 2 ndi/4e | |
10 | seramu dilution microplate | 1 ndi/2e | |
11 | Malangizo | 1 pcs |