Chidule | Kuzindikira kwa antibody ya Bovine Tuberculosis (BTB). |
Mfundo yofunika | Bovine Tuberculosis(BTB) antibody Elisa test kit ingagwiritsidwe ntchito kuzindikira ma antibody a Bovine Tuberculosis mu seramu kapena plasma ya Bovine. |
Zolinga Zozindikira | Ma antibodies a Bovine Tuberculosis (BTB). |
Chitsanzo | Seramu
|
Kuchuluka | 1 kit = 192 Mayeso |
Kukhazikika ndi Kusunga | 1) Ma reagents onse ayenera kusungidwa pa 2 ~ 8 ℃.Osaundana. 2) Nthawi ya alumali ndi miyezi 12.Gwiritsani ntchito ma reagents onse tsiku lomaliza ntchito lisanafike.
|
Mycobacterium bovis ndi mabakiteriya omwe amakula pang'onopang'ono (16- mpaka 20-hour generation time) aerobic bacterium ndi causative agent wa chifuwa chachikulu cha ng'ombe (chodziwika kuti bovine TB).Zimagwirizana ndi Mycobacterium tuberculosis, bakiteriya yomwe imayambitsa chifuwa chachikulu mwa anthu.M. bovis amatha kulumpha chotchinga cha mitundu ndikuyambitsa matenda a chifuwa chachikulu mwa anthu ndi nyama zina zoyamwitsa.
Izi zida ntchito mosalunjika ELISA njira, oyera BTB antigen is atakutidwa kale on enzyme micro - bwino mizere. Poyesa, onjezani kuchepetsedwa seramu chitsanzo, pambuyo incubation, if Apo is BTB mwachindunji antibody, it adzatero phatikiza ndi ndi atakutidwa kale antigen, kutaya ndi osaphatikizidwa antibody ndi zina zigawo ndi kusamba; ndiye onjezani enzyme conjugate, kutaya ndi osaphatikizidwa enzyme conjugate ndi
kusamba. Onjezani gawo lapansi la TMB mu zitsime zazing'ono, chizindikiro cha buluu ndi Enzyme catalysis ndicholunjika kuchuluka kwa ma antibodies mu zitsanzo.
Reagent | Voliyumu 96 Mayesero/192Mayeso | ||
1 |
| 1 ndi/2e | |
2 |
| 2.0ml | |
3 |
| 1.6ml pa | |
4 |
| 100 ml | |
5 |
| 100 ml | |
6 |
| 11/22 ml | |
7 |
| 11/22 ml | |
8 |
| 15ml | |
9 |
| 2 ndi/4e | |
10 | seramu dilution microplate | 1 ndi/2e | |
11 | Malangizo | 1 pcs |