Zogulitsa
Mazira Drop Syndrome1976 kachilombo ka Antibody ELISA Ki
Mazira Drop Syndrome1976 kachilombo ka Antibody ELISA Ki
| Chidule | Use kuti azindikire antibody yeniyeni yolimbana ndi EDS76 mu seramu moyenera. |
| Mfundo yofunika | Kachilombo ka Eggs Drop Syndrome 1976 (EDS76) Ab Elisa amagwiritsidwa ntchito pozindikira antibody yeniyeni yolimbana ndi EDS76 mu seramu moyenera.Poyang'anira ma antibody pambuyo pa EDS76 immune and serological diagnostic of infection in Avian. |
| Zolinga Zozindikira | Aantibody motsutsana ndi EDS76 mu seramu |
| Chitsanzo | Seramu
|
| Kuchuluka | 1 kit = 192 Mayeso |
|
Kukhazikika ndi Kusunga | 1) Ma reagents onse ayenera kusungidwa pa 2 ~ 8 ℃.Osaundana. 2) Nthawi ya alumali ndi miyezi 12.Gwiritsani ntchito ma reagents onse tsiku lomaliza ntchito lisanafike.
|
Zambiri
Egg Drop Syndrome (EDS-76) ndi matenda opatsirana omwe amayamba chifukwa cha adenovirus gulu III la Adenoviridae Avian Virus genus ndi hemagglutination.M’mafamu ena a nkhuku, mazira ochuluka a nkhuku anachepa mwadzidzidzi, ndipo mazira opunduka monga mazira a zipolopolo zofewa, mazira opanda zipolopolo, ndi mazira a zipolopolo zopyapyala anapangidwa nthawi yomweyo.Matenda onsewa amatha kwa masabata 5-6, pambuyo pake kuchuluka kwa mazira kumawonjezeka pang'onopang'ono, koma n'zovuta kufika pamlingo usanachepetse.
Mfundo ya Mayeso
Chidachi chimagwiritsa ntchito njira ya ELISA yosalunjika, antigen yoyeretsedwa ya EDS76 imakutidwa pamizere yaying'ono ya chitsime cha enzyme.,ngati pali EDS76ma virus enieni amtundu wake, iphatikiza ndi antigen yomwe idakutidwa kale, kutaya ma antibody osaphatikiza ndi zigawo zina ndikutsuka;kenaka yikani enzyme yolembedwa kuti anti-EDS76 virus monoclonal antibody, ndiye kuphatikiza kwa anti-monoclonal antibody ndi antijeni yokutira kale;kutaya enzyme yosakanikirana ndi kuchapa;Onjezani gawo laling'ono la TMB mu zitsime zazing'ono, chizindikiro cha buluu chopangidwa ndi Enzyme catalysis chili mugawo losiyana la antibody zomwe zili mu zitsanzo, gwiritsani ntchito owerenga ELISA pa 450nm wavelenth kuti muyese kuyamwa kwa A phindu mu zitsime zomwe zimayankhidwa mutawonjezera njira yoyimitsa.
kuti asiye kuchitapo kanthu.
Zamkatimu
| Reagent | Voliyumu 96 Mayesero/192Mayeso | ||
| 1 |
| 1 ndi/2e | |
| 2 |
| 2.0ml ku | |
| 3 |
| 1.6ml pa | |
| 4 |
| 100 ml | |
| 5 |
| 100 ml | |
| 6 |
| 11/22 ml | |
| 7 |
| 11/22 ml | |
| 8 |
| 15ml ku | |
| 9 |
| 2 ndi/4e | |
| 10 | seramu dilution microplate | 1 ndi/2e | |
| 11 | Malangizo | 1 pcs |
