Chidule | Kuzindikirika kwa ma antibody oletsa anti-bursa of Fabricius virus mu seramu ya nkhuku |
Zolinga Zozindikira | Chicken matenda a bursal virus antibody |
Chitsanzo | Seramu
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Kuchuluka | 1 kit = 192 Mayeso |
Kukhazikika ndi Kusunga | 1) Ma reagents onse ayenera kusungidwa pa 2 ~ 8 ℃.Osaundana. 2) Nthawi ya alumali ndi miyezi 12.Gwiritsani ntchito ma reagents onse tsiku lomaliza ntchito lisanafike.
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Matenda a bursal opatsirana(IBD), yomwe imadziwikanso kuti Gumboro disease, infectious bursitis ndi infectious avian nephrosis, ndi matenda opatsirana kwambiri a achinyamata.nkhukundi turkeys zoyambitsidwa ndi matenda opatsirana a bursal virus (IBDV), omwe amadziwika ndiimmunosuppressionndi kufa nthawi zambiri pazaka 3 mpaka 6 zakubadwa.Matendawa adapezeka koyamba muGumboro, Delawaremu 1962. Ndizofunikira kwambiri pazachuma kumakampani a nkhuku padziko lonse lapansi chifukwa chakuchulukirachulukira ku matenda ena komanso kusokoneza kothandiza.katemera.M'zaka zaposachedwa, mitundu yowopsa kwambiri ya IBDV (vvIBDV), yomwe imayambitsa kufa kwa nkhuku, yayamba ku Europe,Latini Amerika,South-East Asia, Africa ndiKuulaya.Kutenga kachilomboka kumachitika kudzera munjira ya oro-fecal, ndipo mbalame zomwe zakhudzidwa zimatulutsa kachilombo ka HIV pafupifupi milungu iwiri mutadwala.Matendawa amafala mosavuta kuchoka ku nkhuku zopatsirana kupita ku nkhuku zathanzi kudzera mu chakudya, madzi komanso kukhudzana.
The zida amagwiritsa mpikisano ELISA njira, chisanadze mmatumba matenda bursal matenda VP2 mapuloteni pa microplate, ndi kupikisana ndi odana ndi VP2 mapuloteni oteteza thupi mu seramu kwa olimba gawo vekitala ntchito odana ndi VP2 mapuloteni monoclonal antibody.Pakuyezetsa, anti-monoclonal antibody kuti ayesedwe ndi anti-VP2 puloteni amawonjezeredwa, ndipo pambuyo pa incubation, ngati chitsanzocho chili ndi nkhuku yopatsirana matenda a bursal virus VP2 mapuloteni enieni a antibody, amamangiriza ku antigen pa mbale yokutidwa.Potero kutsekereza kumanga kwa odana ndi VP2 mapuloteni monoclonal antibody kwa antigen, pambuyo kutsuka kuchotsa unbound antibody ndi zigawo zina;kenako ndikuwonjezera anti-mbewa enzyme-yolemba antibody yachiwiri kuti imangirire ku antigen-antibody complex pa mbale yozindikira;The unbound enzyme conjugate imachotsedwa ndi kuchapa;gawo laling'ono la TMB limawonjezedwa ku microwell kuti likhale ndi mtundu, ndipo kuyamwa kwachitsanzo kumayenderana molakwika ndi zomwe zili mu anti-VP2 protein antibody zomwe zili mmenemo, potero kukwaniritsa cholinga chozindikira anti-VP2 protein antibody mu chitsanzo.
Reagent | Voliyumu 96 Mayesero/192Mayeso | ||
1 |
| 1 ndi/2e | |
2 |
| 2.0ml ku | |
3 |
| 1.6ml pa | |
4 |
| 100 ml | |
5 |
| 100 ml | |
6 |
| 11/22 ml | |
7 |
| 11/22 ml | |
8 |
| 15ml ku | |
9 |
| 2 ndi/4e | |
10 | seramu dilution microplate | 1 ndi/2e | |
11 | Malangizo | 1 pcs |