Chidule | Kuzindikira kwa Antibody yeniyeni ya Flu Kuzindikira kwa chitetezo chamthupi ndi serological cha matenda mu Avian, nkhumba ndi Equus. |
Mfundo yofunika | Influenza A antibody Elisa kit amagwiritsidwa ntchito kuti azindikire ma antibody omwe amatsutsana ndi INfluenza A Virus (Flu A) mu seramu, yowunikira ma antibody pambuyo pa Flu A chitetezondi serological matenda a matenda mu Avian, nkhumba ndi Equus.
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Zolinga Zozindikira | Influenza A antibody |
Chitsanzo | Seramu
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Kuchuluka | 1 kit = 192 Mayeso |
Kukhazikika ndi Kusunga | 1) Ma reagents onse ayenera kusungidwa pa 2 ~ 8 ℃.Osaundana. 2) Nthawi ya alumali ndi miyezi 12.Gwiritsani ntchito ma reagents onse tsiku lomaliza ntchito lisanafike.
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Influenza A ndi tizilombo toyambitsa matenda timene timayambitsa fuluwenza mu mbalame ndi nyama zina zoyamwitsa.Ndi kachilombo ka RNA, kamene kamakhala kosiyana ndi mbalame zakutchire.Nthawi zina, imafalikira kuchokera ku mbalame zakutchire kupita ku nkhuku, zomwe zingayambitse matenda aakulu, miliri, kapena miliri ya chimfine.
Chidachi chimagwiritsa ntchito njira ya ELISA, antigen ya FluA imakutidwa kale pa microplate.Poyesa, onjezani chitsanzo cha seramu yochepetsedwa, mutatha kukulitsidwa, ngati pali chimfine Antibody yeniyeni, idzaphatikizana ndi antigen yophimbidwa kale, kutaya anti-antibody yosaphatikizana ndi zigawo zina ndikutsuka;kenako onjezerani enzyme yolembedwa kuti anti-Flu Antibody monoclonal, antibody mu zitsanzo zotchinga kuphatikiza kwa monoclonal antibody ndi antijeni wokutira kale;kutaya enzyme yosaphatikizana yolumikizana ndi kutsuka. Onjezani gawo laling'ono la TMB mu zitsime zazing'ono, chizindikiro cha buluu chopangidwa ndi Enzyme catalysis chili mugawo losiyana la zomwe zili mu zitsanzo.
Reagent | Voliyumu 96 Mayesero/192Mayeso | ||
1 |
| 1 ndi/2e | |
2 |
| 2.0ml ku | |
3 |
| 1.6ml pa | |
4 |
| 100 ml | |
5 |
| 100 ml | |
6 |
| 11/22 ml | |
7 |
| 11/22 ml | |
8 |
| 15ml ku | |
9 |
| 2 ndi/4e | |
10 | seramu dilution microplate | 1 ndi/2e | |
11 | Malangizo | 1 pcs |