Brucella Ag Test Kit | |
Nambala yakatalogi | Mtengo wa RC-CF10 |
Chidule | Kuzindikira kwa antigen yeniyeni ya Brucella mkati mwa mphindi 10 |
Mfundo yofunika | Gawo limodzi la immunochromatographic assay |
Zolinga Zozindikira | Brucella Antigen |
Chitsanzo | Canine, bovine ndi Ovis Magazi Onse, Plasma kapena Seramu |
Nthawi yowerenga | 10-15 mphindi |
Kumverera | 91.3% motsutsana ndi IFA |
Mwatsatanetsatane | 100.0% motsutsana ndi IFA |
Malire Ozindikira | IFA Titer 1/16 |
Kuchuluka | 1 bokosi (zida) = 10 zida (payekha payokha) |
Zamkatimu | Zida zoyesera, machubu, zotsitsa zotaya |
Chenjezo | Gwiritsani ntchito mkati mwa mphindi 10 mutatsegula Gwiritsani ntchito sampuli yoyenera (0.01 ml ya dropper) Gwiritsani ntchito pambuyo pa mphindi 15-30 ku RT ngati zasungidwa m'malo ozizira Ganizirani zotsatira za mayeso ngati zosavomerezeka pakatha mphindi 10 |
Mtundu wa Brucella ndi membala wa banja la Brucellaceae ndipo umaphatikizapo mitundu khumi yomwe ili yaing'ono, yosasunthika, yopanda sporing, aerobic, gram-negative intracellular coccobacilli.Iwo ndi catalase, oxidase ndi urea positive mabakiteriya.Mamembala amtundu amatha kukulira pazofalitsa zolemeretsedwa monga magazi agar kapena chokoleti agar.Brucellosis ndi zoonosis yodziwika bwino, yomwe imapezeka m'makontinenti onse, koma ndi kufalikira kwakukulu ndi zochitika, mu zinyama ndi anthu.Brucella, monga tizilombo toyambitsa matenda, timapanga mitundu yambiri ya nyama zomwe zimakhalira nthawi zonse, mwina kwamuyaya, mwina kwa moyo wawo wonse.
Gulu la Brucella maonekedwe
Mitundu ya Brucella nthawi zambiri imafalikira pakati pa nyama pokhudzana ndi placenta, fetus, madzi a fetal ndi kutuluka kwa nyini.nyama yodwala.Mitundu yambiri kapena yonse ya Brucella imapezekanso mu umuna.Amuna amatha kutaya zamoyozi kwa nthawi yayitali kapena moyo wonse.Mitundu ina ya Brucella yapezekanso m'mitsempha ndi zotuluka zina kuphatikiza mkodzo, ndowe, hygroma fluid, salvia, mkaka ndi m'mphuno ndi ocular secretions.
Ecology ya matenda a zoonotic Brucella
Mu ng'ombe
Palibe njira yabwino yodziwira nyama zomwe zili ndi kachilombo ndi maonekedwe awo.Zizindikiro zodziwikiratu pazirombo zapakati ndizochotsa mimba kapena kubadwa kwa ng'ombe zofooka.Kupanga mkaka kungachepe chifukwa cha kusintha kwa nthawi yoyamwitsa yomwe imabwera chifukwa chochotsa mimba komanso kuchedwa kutenga pakati.Zizindikilo zina za brucellosis ndi monga kutsika kwa mphamvu ya kubala ndi kusabereka bwino, kusabereka pambuyo pobereka ndi matenda obwera chifukwa cha uterine, komanso (nthawi zina) kukulitsa mafupa a nyamakazi.
Mu agalu
Mwa agalu, mabakiteriya a Brucellosis nthawi zambiri amakhala pansi pa maliseche ndi lymphatic system, koma amatha kufalikira ku impso, maso ndi intervertebral disc.Pamene brucellosis imakhudza intervertebral disc, zotsatira zake ndi discopondylitis.Kwa agalu, zizindikiro zochokera ku ziwalo zoberekera ndizofala.Mwachitsanzo, agalu aamuna amatha kuyambitsa kutupa kwa scrotal ndi testicular, pomwe agalu achikazi amatha kupita padera.Kutentha thupi sikozolowereka, koma kupweteka kwa Brucellosis kungapangitse galu kukhala wofooka.Ngati matendawa afalikira ku impso, maso kapena zizindikiro za intervertebral disc zingayambe kusonyeza kuchokera ku ziwalozi.
Mu nkhumba
Nthawi yapakati pa matenda ndi maonekedwe a zizindikiro za matenda amatha kuyambira sabata imodzi mpaka miyezi iwiri.Zizindikiro zosonyeza kuti ng'ombe zatenga kachilomboka ndizovuta kwambiri kubereka - kuchotsa mimba, kubwereranso kuntchito pambuyo poti zikwere komanso kubadwa kwa ana a nkhumba ofooka kapena akufa.Ng'ombe zina zimatha kukhala ndi matenda a chiberekero ndikuwonetsa kumaliseche.Anguluwe omwe ali ndi kachilomboka amatha kutupa, machende otupa.Amuna ndi akazi onse amatha kukhala olumala ndi kutupa mafupa ndi/kapena kukhala ndi zizindikiro za kusalumikizana ndi kulumala kwa mwendo wakumbuyo.
1.Kudzipatula ndikuzindikiritsa wothandizira
Mitundu ya Brucella ingathe kupezedwa kuchokera ku minofu ndi zotuluka zambiri, makamaka minyewa ya mwana wosabadwayo, kutulutsa kwa nyini, mkaka (kapena kutulutsa mawere), umuna, nyamakazi yamadzimadzi a hygroma, komanso m'mimba, ndulu ndi mapapo kuchokera kwa ana ochotsedwa.Mitundu yambiri ya Brucella yochokera m'madera mkati mwa masiku ochepa pazosankha zosankhidwa.Pamene mbale zimawonedwa masana kudzera mandala sing'anga, madera amenewa translucent ndi wotumbululuka uchi mtundu.Tikayang'ana pamwamba, maderawo amaoneka ngati otukumula komanso oyera ngati ngale.Kenako maderawo amakhala okulirapo komanso akuda pang'ono.
2.Nucleic acid Njira
PCR ndi njira yabwino yodziwira matenda a brucellosis.Mayesero ambiri a PCR apangidwa kuti azindikire Brucella kuti apititse patsogolo luso lozindikira.Kuyesa kwamtundu wa PCR ndikokwanira kuzindikirika kosavuta kwa Brucella.
3.Kuzindikira kwa serological
Pali mayeso ambiri a serological.Mayeso a serological omwe amagwiritsidwa ntchito poyesa ng'ombe kapena ng'ombe pawokha amaphatikiza kuyesa kwa Brucella antigen, kuwongolera kowonjezera, ma enzyme-linked immunosorbent assays(ELISA) ndi fluorescence assay.